The Visionary - Spring 2015 - page 5

Lions Eye Institute Newsletter |5
These treatments reduce the pressure
and, inmost cases, delay the death of
the retinal ganglion cells. The TM is
where the outflow is impeded causing
the pressure increase. If we can learn
more about changes in the TM or
help develop a compound that would
increase the outflow, perhaps more
people would not have to lose their
sight to this disease.
The Ocular Research Teamhas
set up a model system that will allow
us to pursue these goals. The model,
originally developed at the Mayo
clinic a number of years ago, consists
of a computer machined plastic dish
and the dissected front of a human
eye held in place and infused with
cell culture media. The media flows
into the inside of the systemand exits
via through the same tissue route
that aqueous humor does in the
intact eye. Once the initial infusion
of media is completed, the dish is
connected to a specialized pump
that can infuse media in
at the same rate as natural
aqueous humor production
(2.5 µL or 0.0025 mL
per minute). The other
component of the system
is a specialized pressure
sensing device (transducer)
that is connected to a computer that
records the internal pressure once
every minute over the course of
several days.
This model will allow us to
collaborate with academic partners
in order to examine changes in the
TM. In addition, pharmaceutical
industry collaborators want to test
new compounds to try to lower the
intraocular pressure by affecting the
TM. LEITR scientists will generate
pressure data, collectmedia
that passes through the
TM to examine changes
in proteins and preserve
tissue for morphological
studies. The ability to
acquire high quality ocular
research tissue is a key
factor for the success of this model.
LEITR is the leader in supplying high
quality ocular research tissue so the
research scientists here are poised
to use this model to explore, “The
science of sight.”
AshleyMorganti checks a POC dish
to ensure that themedia supply line and
the pressure transducer line are correct
prior to placing it in the incubator. The
pressure transducers for the dishes are
attached to the side incubator (left).
Syringes, with blue filtering disks and
containingcell culturemediaare clamped
into the perfusion pump which infuses
themedia at a rate of 2.5 µL/minute into
the dishes.
The ORC Team
(Left to Right) Nicholas Sprehe, Ashley Morganti and Dr. Mitch
McCartney stand in front of the computer that records the pressure readings for the perfusion
organ culture (POC) model. The system records a reading everyminute over the course of an
experiment which lasts from 3 to 7 days.
2.3 million
over the age
of 40 have
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find a cure!
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